Course Catalog

Advanced HPLC/UHPLC 1: Fundamentals Online Short Course

This course will provide the attendees with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns and pharmaceutical applications of modern HPLC and UHPLC (ultra-high-pressure liquid chromatography).

Course Details

Key Topics

  • History, advantages, limitations, and modes
  • HPLC columns, trends and selection guides
  • HPLC instrumentation and operating principles
  • Practical applications of HPLC in diversified industries


This course will provide you with an updated overview and a solid working knowledge of high-performance liquid chromatography (HPLC). The attendees will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guide, and key applications in various industries. This is the first part of a two-course series for Advanced HPLC/UHPLC. The second part, “Advanced HPLC/UHPLC 2:  Pharmaceutical Applications and UHPLC Concepts, Practices and Potential Issues Online Course”, is a follow-on course that focuses on the practice of HPLC: operation, maintenance, troubleshooting, method development as well as recent developments of ultra-high-pressure LC.

M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006 (ISBN-10: 047172789X).

Who Should Attend

Analysts, scientists, researchers, and managers in pharmaceutical and biopharmaceutical companies who want to get an updated overview of fundamentals and best practice of modern HPLC/UHPLC in pharmaceutical analysis. A basic understanding of chemistry and HPLC with some hands-on experience is assumed.


The attendees will get a critical overview of the fundamentals of modern HPLC/UHPLC relevant in pharmaceutical analysis including modern concepts, advances in instruments, and latest column trends and selection guide.


Modern Concepts

  • Introduction: the chromatographic process, advantages and limitations, and chromatographic modes
  • Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs), tailing factor, the resolution equation, linear solvent strength theory, and column void volume (VM)
  • Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG), column temperature (T)), peak capacity (Pc), and references

HPLC Columns, Trends and Selection Guides

  • Column characteristics and types, packing characteristics (support type, particle size, pore size) and bonded phases, end-fittings, van Deemter equation and estimation of column efficiency
  • Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, concept of “orthogonality”, listing of RPC columns, and column selection guide
  • Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2mm, and superficially porous particles (SPP)

HPLC instrumentation and Operating Principles

  • Integrated vs. modular systems, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume and ramification, modern pump trends, manual injection valve, and autosampler types and trends
  • Detectors: Operating principles of UV/Vis detection and characteristics, wavelength selection, photodiode array detector, newer detectors for non-chromophoric analytes (ELSD, CAD and CLND), applications of CAD
  • Mass spectrometer (MS), types of MS and ESI interface, data handling system (CDS). steps in sample analysis, concept of instrumental bandwidth (extra-column bandbroadening), loss of column efficiency vs. column size

Course Locations


October 11 - 25, 2017

Each session runs 10:30AM-12:30 PM EST

This course will meet online for 3 sessions from 10:30 am to 12:30 pm ET on the following dates:

October 11
October 18
October 25

Attendance at scheduled class times is NOT required. Registered participants can attend the course by viewing session recordings.

Register Online Register Via Mail



Each person attending must register individually for this course

The course fee includes a electronic access to the course materials.

System Requirements
You need a high-speed connection to the Internet and an up-to-date version of your web browser. The audio portion of the live session will be provided via VOIP and US toll-free conference call. VOIP is dependent on the quality and speed of your internet connection so a high-speed connection is strongly recommended. Those choosing to use VOIP should also have a headset or microphone in order to interact with the instructor. If not, we recommend you choose the telephone option, or contact us to purchase a headset for $5.

  Member Non-Member
Standard $795 per person $995 per person

Four for three! Register three people for one online course, one person for three online courses, or any combination in between, and the fourth registration is free! This offer can only be used in conjunction with online registrations and cannot be combined with any other offer. All courses must be completed in the same calendar year. All four registrations must be received by our offices on the same day. Note: This discount is only available if you register by fax, mail or phone and mention this discount.

About the Instructor

  • Michael W. Dong

    is a principal in MWD Consulting. He was formerly Senior Scientist at Genentech, Research Fellow at Purdue Pharma, and Staff Scientist at Perkin-Elmer. He holds a Ph.D. in Analytical Chemistry from City University of New York and has 100+ publications and 3 books.