Course Catalog

Modern HPLC/UHPLC for Practicing Scientists 2: UHPLC, Method Development, HPLC Operation, and Troubleshooting Online Course

An overview of separation science in pharmaceutical discovery and development, high-throughput purification, preclinical research and chemistry, manufacturing and control (CMC), formulation, the role of the analytical chemist, techniques for physicochemical characterization

Course Details

Key Topics

  • Operation
  • Maintenance and Troubleshooting
  • Method Development and Validation
  • Novel approaches and recent advances in HPLC

Information

This intermediate 3-session webinar will provide the analytical scientist with a clearer understanding and a solid working knowledge of the best practices in UHPLC, method development, and HPLC operation/troubleshooting.

 

Recommended textbook:

W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, Hoboken, New Jersey, 2006 (ISBN-10: 047172789X). List price at $92 and available from Amazon or John Wiley.

This book is used as a course reference and supplements the course presentation handouts.

 

 

Who Should Attend

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview on the best practice of modern HPLC/UHPLC in method development, operation/troubleshooting and other applications (Food, environmental, chemical, biosciences, and biotherapeutics). A basic understanding of chemistry and HPLC with some hands-on experience is assumed.

Benefits

The attendee will get a critical overview of the applications of separation science from drug discovery, analytical develop to quality control including the best practice in small molecule drug development and the analysis of recombinant biologics. The attendee will also learn the benefits, best practices and potential issue of UHPLC in pharmaceutical analysis.

Recommended textbook:

M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006 (ISBN-10: 047172789X).

Agenda

Modern (u)HPLC 2: UHPLC, Method Development, and Operation/Troubleshooting

1.UHPLC: Concept, Perspectives, Practice and Potential Issues

A. Overview and perspectives, UHPLC concepts (the need for high pressure, void volume, peak volume, instrumental bandwidth), UV flow cell design, dwell volume

B. Benefits and case studies: very fast separations with good resolution, very high-resolution analysis of complex samples, facilitating rapid method development, flexibility to customize resolution

C. Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC and vice versa using geometric scaling, how to transition from HPLC to UHPLC, end-fittings

2. HPLC Method Development and Validation

A. Tradition strategy for HPLC method development

B. 3-pronged template approach for rapid method development

C. Use of a universal generic gradient method for the assay of multiple NCEs and stability-indicating assays with simple adjustments

D. A brief overview of method validation

3.HPLC Operation and Troubleshooting

A. Mobile phase and sample preparation and best practice in HPLC operation

B. Problem diagnosis and troubleshooting guide with case studies

C. Diagnosing and solving problems (pressure, baseline, peak, data performance)

Course Locations

Date

November 1 - 15, 2018

Thursdays
11:30 AM – 1:30 PM
November 1
November 8
November 15

Register Online Register Via Mail

Venue

Online

Each person attending must register individually for this course

The course fee includes a electronic access to the course materials.

Four for three! Register three people for one online course, one person for three online courses, or any combination in between, and the fourth registration is free! This offer can only be used in conjunction with online registrations and cannot be combined with any other offer. All courses must be completed in the same calendar year. All four registrations must be received by our offices on the same day. Note: This discount is only available if you register by fax, mail or phone and mention this discount.


Pricing
  Member Non-Member
Standard $895 $1095

System Requirements
You need a high-speed connection to the Internet and an up-to-date version of your web browser. The audio portion of the live session will be provided via VOIP and US toll-free conference call. VOIP is dependent on the quality and speed of your internet connection so a high-speed connection is strongly recommended. Those choosing to use VOIP should also have a headset or microphone in order to interact with the instructor. If not, we recommend you choose the telephone option, or contact us to purchase a headset for $5.

About the Instructor

  • Michael W. Dong

    is a principal consultant in MWD Consulting focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis and drug quality.