Course Catalog

Capillary Electrophoresis of Biomolecules

Dr. Weinberger has taught this course over 20 years to thousands of students. You will learn how to develop methods and implement CE for the separation of biomolecules. The course begins with an extensive review describing the factors that influence the electrophoretic migration of molecules in fluid solution. A phenomenological approach is employed that enables the student to take home a thorough understanding of electrophoretic processes. The curriculum includes:

  • Modules covering the modes of capillary electrophoresis
  • Capillary zone electrophoresis including micellar electrokinetic separations
  • Capillary isoelectric focusing
  • Capillary sieving electrophoresis (size separations)
  • Methods development using each of the CE modes
  • Applications including amino acids, peptides and proteins, SDS-proteins and carbohydrates
  • If there is sufficient interest, DNA applications including oligonucleotides, sequencing, plasmids, short-tandem repeats and genetic analysis
  • Troubleshooting

Course Details

Key Topics

  • How to perform quantitative analysis
  • How to develop robust methods
  • Sample preparation methods for CE
  • Why dynamic coatings are important
  • The advantages and disadvantages of each CE injection technique
  • Various detection techniques such as UV, LIF and mass spectrometry
  • Why on-capillary detection is different from post-column detection
  • How to logically troubleshoot a malfunctioning method
  • How to implement stacking procedures to overcome sensitivity problems
  • How to perform method validation, methods transfer and failure analysis
  • The types and availability of commercial equipment
  • Why microfabricated systems represent the next generation of CE instruments

Information

Dr. Weinberger has taught this course over 20 years to thousands of students. You will learn how to develop methods and implement CE for the separation of biomolecules. The course begins with an extensive review describing the factors that influence the electrophoretic migration of molecules in fluid solution. A phenomenological approach is employed that enables the student to take home a thorough understanding of electrophoretic processes. The curriculum includes:

  • Modules covering the modes of capillary electrophoresis
  • Capillary zone electrophoresis including micellar electrokinetic separations
  • Capillary isoelectric focusing
  • Capillary sieving electrophoresis (size separations)
  • Methods development using each of the CE modes
  • Applications including amino acids, peptides and proteins, SDS-proteins and carbohydrates
  • If there is sufficient interest, DNA applications including oligonucleotides, sequencing, plasmids, short-tandem repeats and genetic analysis
  • Troubleshooting

Who Should Attend

The course is designed for analytical chemists and analytical biochemists and those in related fields who wish to implement Capillary Electrophoresis in their laboratories. While appropriate for novice users, the course will especially benefit experienced users who wish to broaden their background.

  • Scientists from R&D, QC, methods transfer and process control groups from biotechnology and biopharmaceutical companies
  • Graduate students beginning CE projects will get a jump-start for their research.

Benefits

  • Develop confidence in your ability to develop methods and implement Capillary Electrophoresis (CE) in your laboratory
  • Understand the unique issues concerning quantitative analysis with CE
  • Benchmark your CE projects with colleagues from similar organizations that have a similar set of problems
  • Understand why training is so important when implementing CE
  • Learn where to locate the best sources of technical information

Agenda

  • Introduction to capillary electrophoresis
    • The transition from slab-gels to capillaries
    • Using diagrams to help understand the impact of ion migration on separations
    • How to optimize resolution
    • Buffers and buffer additives
    • Commercial instrumentation
  • Capillary zone electrophoresis
    • Effective use of mobility plots
    • How to control wall effects
    • How to develop a well controlled separation
    • When to use and how to optimize secondary equilibrium and micellar electrokinetic separations
    • Case study, heparin
  • Capillary isoelectric focusing
    • Introduction to isoelectric focusing
    • How to select instruments, capillaries and ampholytes
    • Case study, erythropoietin
  • Capillary sieving electrophoresis
    • Introduction to polymer networks
    • Method development strategies
    • Size separations for proteins and DNA
    • Case study, monoclonal antibodies
  • Injection
    • When to use and how to optimize both electrokinetic and hydrodynamic injection
  • Detection
    • Why corrected peak areas are required with CE
    • Types of detection including mass spectrometry (MS)
    • Case study, carbohydrate separations with detection by laser induced fluorescence and MS
  • Troubleshooting guide
    • Common and unusual problems are described
  • Stacking and trace enrichment
    • How to optimize stacking to solve sensitivity problems
  • Microfabricated systems
    • Why microfabricated systems represent the next generation of CE
  • Validation and methods transfer
    • Experiences at large biotech companies
    • Case studies
    • Failure analysis

Course Locations

Date

TBA

Check-in opens at 7:30 a.m. on the first day of the course.

Course runs from 8:30 a.m. to 5:00 p.m. each day.

Register Via Mail

Venue

The course fee includes a course binder and a continental breakfast each day.

Five for Four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. Note: This discount is only available if you register by fax, mail or phone and mention this discount. This discount may not be combined with any other offer.


Pricing
  Member Non-Member
Advanced $1,695 $1,895
Standard $1,895 $2.095

About the Instructor