Course Catalog

Essentials of Modern HPLC/UHPLC 1: Fundamentals and Applications

This course will provide you with an updated overview and a solid working knowledge of high-performance liquid chromatography (HPLC). The attendees will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guide, and key applications in various industries. This is the first part of a two-course series for Essential of Modern HPLC/UHPLC. The second part, “Essential of Modern HPLC/UHPLC 2”, is a follow-on course that focuses on the practice of HPLC: operation, maintenance, troubleshooting, method development as well as recent developments of ultra-high-pressure LC.

Recommended Textbook: M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Course Details

Key Topics

  • History, advantages, limitations, and modes
  • HPLC columns, trends and selection guides
  • HPLC instrumentation and operating principles
  • Practical applications of HPLC in diversified industries

Information

This course will provide you with an updated overview and a solid working knowledge of high-performance liquid chromatography (HPLC). The attendees will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guide, and key applications in various industries. This is the first part of a two-course series for Essential of Modern HPLC/UHPLC. The second part, “Essential of Modern HPLC/UHPLC 2”, is a follow-on course that focuses on the practice of HPLC: operation, maintenance, troubleshooting, method development as well as recent developments of ultra-high-pressure LC.

Recommended Textbook: M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Who Should Attend

Analysts, scientists, researchers, and managers who want to get an updated introduction of modern HPLC fundamentals and applications. The focus is to provide the attendees with a concise overview of this common analytical technique and how to apply it to solve practical problems. It is recommended that you have a good understanding of general chemistry. Some prior hands-on HPLC experience would be helpful. This course will be conducted at an intermediate level.

Benefits

  • Obtain an update and overview of modern HPLC and UHPLC pertinent to new users or practicing scientists
  • Learn essence of HPLC: modes, corollaries, mobile phase and basic concepts
  • Learn fundamentals in column characteristics and selection guide
  • Learn HPLC instrumentation and operating principles
  • Learn practical applications in diversified industries including Fast LC and UHPLC case studies

Agenda

  1. Introduction and fundamental concepts
    • History, advantages, limitations, and modes
    • Retention time (tR), retention factor (k), separation factor (±), column efficiency (N), column void volume (VM), and resolution (Rs)
    • Mobile phase factors (organic modifiers, pH, buffers), operating parameters (Flow, Gradient time (tG), column temperature (T), and peak capacity (Pc).)
  2. HPLC columns, trends and selection guides
    • Column characteristics and types, packing characteristics (support type, particle size, pore size) and bonding chemistries
    • Trends of shorter and narrower columns packed with small particles, high-purity silica, novel bonding chemistries
    • van Deemter equation
    • Column selections guide
    • HILIC, monoliths, hybrids, sub-3, sub-2 ¼m and fused-core columns.
  3. HPLC instrumentation and operating principles
    • Solvent delivery system, injector, autosampler, detector (UV/Vis, photodiode array, fluorescence, refractive index, ELSD, CLND, CAD, and mass spectrometer (MS), and data handling system
    • HPLC system calibration
    • Concepts and ramifications of dwell volume and instrumental bandwidth (extra-column bandbroadening).
  4. Practical applications of HPLC/UHPLC in diversified industries
    • An overview of HPLC applications in diversified industries supported with specific case studies
    • Pharmaceutical: drug discovery to quality control, assay, impurities, chiral seperations and dissolution
    • Food: sugars, fats, organic acids, and additives
    • Environmental: US EPA methods, pesticides, and PAHs
    • Chemical: GPC , plastics, and ion-chromatography
    • Bioseparations and life sciences: proteins, peptides, amino acids, oligonucleotides, nuclei acids, and PCR products

Course Locations

Date

TBA

Check-in opens at 7:30 a.m.

Course runs from 8:30 a.m. to 5:00 p.m.

Register Online Register Via Mail

Venue


Pricing
Advanced $550
Standard $850

The course fee includes a course binder and a continental breakfast.

Five for Four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. Note: This discount is only available if you register by fax or mail and mention this discount. May not be combined with any other offer.

About the Instructor

  • Michael W. Dong

    is a principal in MWD Consulting. He was formerly Senior Scientist at Genentech, Research Fellow at Purdue Pharma, and Staff Scientist at Perkin-Elmer. He holds a Ph.D. in Analytical Chemistry from City University of New York and has 100+ publications and 3 books.