Course Catalog

Essentials of Modern HPLC/UHPLC 2: Practice, Operation, Troubleshooting and Method Development Online Short Course

This course will introduce you to best practices of high-performance liquid chromatography (HPLC) and ultra-high-pressure LC (UHPLC) as well as tricks-of-the-trade for successful HPLC operation. The attendees will learn step-by-step guide to operating HPLC modules, maintenance procedures, troubleshooting strategies, traditional and accelerated method development processes as well as recent advances of this pervasive analytical technique including UHPLC. This is the second part of a two-course series to Modern HPLC introduction. “Essentials of Modern HPLC/UHPLC 1″ introduces HPLC fundamental concepts, columns, instrumentations and applications. It is a prerequisite for “Essentials of Modern HPLC/UHPLC 2″ for beginners.

Recommended Textbook: M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Course Details

Key Topics

  • Operation
  • Maintenance and Troubleshooting
  • Method Development and Validation
  • Novel approaches and recent advances in HPLC

Information

This course will introduce you to best practices of high-performance liquid chromatography (HPLC) and ultra-high-pressure LC (UHPLC) as well as tricks-of-the-trade for successful HPLC operation. The attendees will learn step-by-step guide to operating HPLC modules, maintenance procedures, troubleshooting strategies, traditional and accelerated method development processes as well as recent advances of this pervasive analytical technique including UHPLC. This is the second part of a two-course series to Modern HPLC introduction. “Essentials of Modern HPLC/UHPLC 1″ introduces HPLC fundamental concepts, columns, instrumentations and applications. It is a prerequisite for “Essentials of Modern HPLC/UHPLC 2″ for beginners.

Recommended Textbook: M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Who Should Attend

Analysts, scientists, researchers and managers who want to get an updated introduction of modern HPLC practices, troubleshooting, method development and recent advances of UHPLC. You should have a good understanding of general chemistry. Some prior hands-on HPLC/UHPLC experience would be helpful. This course is conducted at an intermediate level.

Benefits

  • Obtain an update or overview on the practice of modern HPLC.
  • Learn step-by-step guide and tricks-of-the-trade in HPLC.
  • Seven common faux pas in modern HPLC.
  • Learn fundamentals of HPLC maintenance and troubleshooting strategies.
  • Learn how to develop and validate HPLC methods.
  • Learn the 3-pronged template approach to rapid HPLC method development.
  • Learn latest development of UHPLC, equipment, benefits, performance, issues and practice.

Agenda

  • HPLC operation, maintenance and troubleshooting
    • Mobile phase preparation
    • Best practice in HPLC operation
    • Guidelines for increasing HPLC precision and for trace analysis
    • Seven common “faux pas” in modern HPLC
    • Common HPLC maintenance procedures
    • Problem diagnosis and troubleshooting guide with case studies
    • Diagnosing diagnosis and troubleshooting guide with case studies
  • HPLC method development and validation
    • Tradition strategy for HPLC method development
    • 3-pronged template approach for efficient method development
    • Method prequalification and validation
    • Modern trends – software tools and automated column/MP screening systems
    • Case studies on developing ICH impurity testing of pharmaceuticals including some discussions on phase-appropriate method development and QbD concepts
  • Ultra high-pressure LC 1  concepts, perspectives, performance and benefits
    • History and current status of UHPLC
    • Practical concepts and instrumental considerations
    • Benefits: very fast separation with good resolution, very high resolution of
      complex samples, facilitating rapid method development
    • Case studies (NCE, OTC, complex molecules and natural products)
  • Ultra high-pressure LC 2  practice, potential issues and QC implementation
    • Potential issues and operating nuances (viscous heating, system dispersion, compatibility to existing methods, injection precision and carryover, detector sensitivity and dwell volumes, and method transfer issues)
    • Case studies illustrating issues and myths, and how to mitigate potential pitfalls
    • How to get started and to transition from HPLC to UHPLC

Course Locations

Date

May 6 - 27, 2015

This course will meet online for 4 sessions from 10:30 am to 12:00 pm ET on the following dates:
Wednesdays
5/6 – 5/27
Attendance at scheduled class times is NOT required. Registered participants can attend the course by viewing session recordings.

Register Online Register Via Mail

Venue

Online

Each person attending must register individually for this course.

The course fee includes a electronic access to the course materials.

4 for 3! Register 4 people for 1 course, 1 person for 4 courses, or any combination in between, and the 4th course is free! This offer can only be used in conjunction with webcast registrations and cannot be combined with any other offer. All courses must be completed in the same calendar year. All 4 registrations must be received by our offices on the same day. Please register by fax or mail and note this offer on your registration form.


Pricing
  Member Non-Member
Standard $795 per person $995 per person

You need a high-speed connection to the Internet and an up-to-date version of your web browser. The audio portion of the live session will be provided via VOIP and US toll-free conference call. VOIP is dependent on the quality and speed of your internet connection so a high-speed connection is strongly recommended. Those choosing to use VOIP should also have a headset or microphone in order to interact with the instructor. If not, we recommend you choose the telephone option, or contact us to purchase a headset for $5.

About the Instructor

  • Michael W. Dong

    is a Senior Scientist at Genentech, Small Molecule Drug Discovery. He teaches Advanced HPLC in Pharmaceutical Analysis and webcast courses Ultra-high-pressure LC (UHPLC): Concepts, Practices, and Issues and How to Get Started; Modern HPLC in Pharmaceutical Analysis; and Modern HPLC for DMPK, Bioanalytical, and Analytical Development Scientists: Essentials, Best Practices.