Course Catalog

Essentials of Modern HPLC/UHPLC 2: Practice, Operation, Troubleshooting and Method Development

This course will introduce you to best practices of high-performance liquid chromatography (HPLC) and ultra-high-pressure LC (UHPLC) as well as tricks-of-the-trade for successful HPLC operation. The attendees will learn step-by-step guide to operating HPLC modules, maintenance procedures, troubleshooting strategies, traditional and accelerated method development processes as well as recent advances of this pervasive analytical technique including UHPLC. This is the second part of a two-course series to Modern HPLC introduction. “Essentials of Modern HPLC/UHPLC 1? introduces HPLC fundamental concepts, columns, instrumentations and applications. It is a prerequisite for “Essentials of Modern HPLC/UHPLC 2? for beginners.

Recommended Textbook: M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Course Details

Key Topics

  • Operation
  • Maintenance and Troubleshooting
  • Method Development and Validation
  • Novel approaches and recent advances in HPLC

Information

This course will introduce you to best practices of high-performance liquid chromatography (HPLC) and ultra-high-pressure LC (UHPLC) as well as tricks-of-the-trade for successful HPLC operation. The attendees will learn step-by-step guide to operating HPLC modules, maintenance procedures, troubleshooting strategies, traditional and accelerated method development processes as well as recent advances of this pervasive analytical technique including UHPLC. This is the second part of a two-course series to Modern HPLC introduction. “Essentials of Modern HPLC/UHPLC 1? introduces HPLC fundamental concepts, columns, instrumentations and applications. It is a prerequisite for “Essentials of Modern HPLC/UHPLC 2? for beginners.

Recommended Textbook: M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Who Should Attend

Analysts, scientists, researchers and managers who want to get an updated introduction of modern HPLC practices, troubleshooting, method development and recent advances of UHPLC. You should have a good understanding of general chemistry. Some prior hands-on HPLC/UHPLC experience would be helpful. This course is conducted at an intermediate level.

Benefits

  • Obtain an update or overview on the practice of modern HPLC.
  • Learn step-by-step guide and tricks-of-the-trade in HPLC.
  • Seven common faux pas in modern HPLC.
  • Learn fundamentals of HPLC maintenance and troubleshooting strategies.
  • Learn how to develop and validate HPLC methods.
  • Learn the 3-pronged template approach to rapid HPLC method development.
  • Learn latest development of UHPLC, equipment, benefits, performance, issues and practice.

Agenda

  • HPLC operation, maintenance and troubleshooting
    • Mobile phase preparation
    • Best practice in HPLC operation
    • Guidelines for increasing HPLC precision and for trace analysis
    • Seven common “faux pas” in modern HPLC
    • Common HPLC maintenance procedures
    • Problem diagnosis and troubleshooting guide with case studies
    • Diagnosing diagnosis and troubleshooting guide with case studies
  • HPLC method development and validation
    • Tradition strategy for HPLC method development
    • 3-pronged template approach for efficient method development
    • Method prequalification and validation
    • Modern trends – software tools and automated column/MP screening systems
    • Case studies on developing ICH impurity testing of pharmaceuticals including some discussions on phase-appropriate method development and QbD concepts
  • Ultra high-pressure LC 1  concepts, perspectives, performance and benefits
    • History and current status of UHPLC
    • Practical concepts and instrumental considerations
    • Benefits: very fast separation with good resolution, very high resolution of
      complex samples, facilitating rapid method development
    • Case studies (NCE, OTC, complex molecules and natural products)
  • Ultra high-pressure LC 2  practice, potential issues and QC implementation
    • Potential issues and operating nuances (viscous heating, system dispersion, compatibility to existing methods, injection precision and carryover, detector sensitivity and dwell volumes, and method transfer issues)
    • Case studies illustrating issues and myths, and how to mitigate potential pitfalls
    • How to get started and to transition from HPLC to UHPLC

Course Locations

Date

May 11, 2017

Check-in opens at 7:30 a.m.

Course runs from 8:30 a.m. to 5:00 p.m.

Register Online Register Via Mail

Venue

Boston University
Metcalf Center for Science and Engineering
590 Commonwealth Avenue Boston, MA 02215


Pricing
Advanced $550
Standard $850

The course fee includes a course binder and a continental breakfast.

Five for Four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. Note: This discount is only available if you register by fax or mail and mention this discount. May not be combined with any other offer.

Date

August 20, 2017

Check-in opens at 7:30 a.m.

Course runs from 8:30 a.m. to 5:00 p.m.

Register Online Register Via Mail

Venue

TBA

Washington, DC 20036


Pricing
Advanced $550
Standard $850

The course fee includes a course binder and a continental breakfast.

Five for Four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. Note: This discount is only available if you register by fax or mail and mention this discount. May not be combined with any other offer.

About the Instructor

  • Michael W. Dong

    is a principal in MWD Consulting. He was formerly Senior Scientist at Genentech, Research Fellow at Purdue Pharma, and Staff Scientist at Perkin-Elmer. He holds a Ph.D. in Analytical Chemistry from City University of New York and has 100+ publications and 3 books.