Course Catalog

Modern HPLC/UHPLC for Practicing Scientists 2: UHPLC, Method Development, HPLC Operation, Troubleshooting and Biopharmaceutical Applications

This intermediate 1-day workshop will provide the analytical scientist with a clearer understanding and a solid working knowledge on the best practices in method development, HPLC operation/troubleshooting, and other applications of modern HPLC and UHPLC including characterization/QC of recombinant biologics.

Recommended Textbook: M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Course Details

Key Topics

  • Operation
  • Maintenance and Troubleshooting
  • Method Development and Validation
  • Novel approaches and recent advances in HPLC

Information

This intermediate 1-day workshop will provide the analytical scientist with a clearer understanding and a solid working knowledge on the best practices in method development, HPLC operation/troubleshooting, and other applications of modern HPLC and UHPLC including characterization/QC of recombinant biologics.

Recommended Textbook: M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Who Should Attend

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview on the best practice of modern HPLC/UHPLC in method development, operation/troubleshooting and other applications (Food, environmental, chemical, biosciences, and biotherapeutics). A basic understanding of chemistry and HPLC with some hands-on experience is assumed.

Benefits

  • Obtain an update or overview on the practice of modern HPLC.
  • Learn step-by-step guide and tricks-of-the-trade in HPLC.
  • Seven common faux pas in modern HPLC.
  • Learn fundamentals of HPLC maintenance and troubleshooting strategies.
  • Learn how to develop and validate HPLC methods.
  • Learn the 3-pronged template approach to rapid HPLC method development.
  • Learn latest development of UHPLC, equipment, benefits, performance, issues and practice.

Agenda

Day 2: Modern (u)HPLC 2: UHPLC, Method Development, Troubleshooting and Other Applications

1.Other HPLC Applications including Recombinant Biologics

  • Food: sugars, fats, organic acids, and additives.
  • Environmental: US EPA methods, pesticides, and PAHs.
  • Chemical: GPC, plastics, and ion-chromatography.
  • Bioseparations and life sciences: proteins, peptides, peptide mapping, amino acids, oligonucleotides, nuclei acids, and PCR products.
  • Characterization and QC of recombinant biologics (Monoclonal Antibodies)

2.UHPLC: Concept, Perspectives, Practice and Potential Issues

  • Overview and perspectives, UHPLC concepts (the need for high pressure, void volume, peak volume, instrumental bandwidth), UV flow cell design, dwell volume
  • Benefits and case studies: very fast separations with good resolution, very high resolution analysis of complex samples, facilitating rapid method development, flexibility to customize resolution
  • Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC and vice versa using geometric scaling, how to transition from HPLC to UHPLC, end-fittings

3.HPLC Method Development and Validation

  • Tradition strategy for HPLC method development
  • 3-pronged template approach for rapid method development
  • Use of a universal generic gradient method for assay of multiple NCEs and stability-indicating assays with simple adjustments
  • A brief overview of method validation

4.HPLC Operation and Troubleshooting

  • Mobile phase and sample preparation and best practice in HPLC operation
  • Problem diagnosis and troubleshooting guide with case studies
  • Diagnosing and solving problems (pressure, baseline, peak, data performance)

Course Locations

Date

April 18, 2018

Check-in opens at 7:30 a.m.

Course runs from 8:30 a.m. to 5:00 p.m.

Register Online Register Via Mail

Venue

Temple University, Center City
1515 Market Street
Philadelphia, PA 19102


Pricing
  Member Non-Member
Advanced $895 (Ends Mar 17) $1095 (Ends Mar 17)
Standard $1095 $1295

The course fee includes a course binder and a continental breakfast.

Five for Four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. Note: This discount is only available if you register by fax or mail and mention this discount. May not be combined with any other offer.

Date

August 19, 2018

Check-in opens at 7:30 a.m.

Course runs from 8:30 a.m. to 5:00 p.m.

Register Online Register Via Mail

Venue

TBA

Boston, MA


Pricing
  Member Non-Member
Advanced $895 (Ends Jul 18) $1095 (Ends Jul 18)
Standard $1095 $1295

The course fee includes a course binder and a continental breakfast.

Five for Four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. Note: This discount is only available if you register by fax or mail and mention this discount. May not be combined with any other offer.

About the Instructor

  • Michael W. Dong

    is a principal consultant in MWD Consulting focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis and drug quality.