Course Catalog

Gas Chromatography: Fundamentals, Troubleshooting, and Method Development

This course is designed for beginners and intermediate-level practitioners who want practical laboratory experience in gas chromatography (GC). The lectures supplemented by problem sets, slides, and video presentations provide the fundamentals needed to understand the technique and instrumentation involved in this powerful analytical tool. At the end of the class, you will have mastered the fundamentals of GC, participated in five hands-on laboratory sessions, performed reference and literature searches, and learned specialized techniques based on your specific interests.

Course Details

Key Topics

  • Overview of Gas Chromatography
  • Injection Techniques
  • Quantitative Analysis
  • Columns and Detectors
  • Temperature Programming
  • Method Development

Information

This course is designed for beginners and intermediate-level practitioners who want practical laboratory experience in gas chromatography (GC). The lectures, supplemented by problem sets, slides, and video presentations, provide the fundamentals needed to understand the technique and instrumentation involved in this powerful analytical tool. At the end of the class, you will have mastered the fundamentals of GC, participated in five hands-on laboratory sessions, performed reference and literature searches, and learned specialized techniques based on your specific interests.

Who Should Attend

This course is designed for beginners and intermediate-level practitioners in gas chromatography who want practical laboratory experience. The lectures, supplemented by problem sets, slides, and video tapes, provide the fundamentals needed to understand the technique and instrumentation involved in this powerful analytical tool.

No previous experience in gas chromatography is required for the course.

Benefits

  • Master the fundamentals of GC
  • Participate in five hands-on laboratory sessions
  • Perform reference and literature searches
  • Learn specialized techniques based on your specific interests

Agenda

Day 1: GC Overview & Injection Techniques

Introduction to GC and instrumentation

The GC Separation:  How it occurs.

Packed & Capillary columns

Capillary inlet systems: split, splitless, on-column

 

Laboratory Experiments:

GC Familiarization / Temperature effects

Split ratio

Comparing split and splitless injection techniques

 

Day 2:  Resolution, Columns and Quantitative Analysis

Resolution Equation (how to choose the correct chromatographic conditions).

GC Theory Overview – Explains why we like small diameters and thin films

Capillary column technology; column dimension and stationary phase selection

Calibration methods:  Area % – internal and external standard

 

Laboratory Experiments:

GC Parameters

Detailed investigation of temperature and flow effects on a separation

 

Day 3: Detectors, GC/MS, Headspace

Overview of available detectors; details of FID & TCD;

GC/MS – How it works and why it is important

Headspace GC – Theory, practice and instrumentation

 

Laboratory Experiments:

Column installation;

Injector disassembly and maintenance;

Quantitation of an unknown employing internal vs. external standard techniques.

 

Day 4:  Method Development & Troubleshooting/Maintenance

Temperature programming;

Fundamentals of method development;

Troubleshooting Fundamentals

Troubleshooting from actual chromatograms

 

Laboratory Experiments:

Develop a method for a completely unknown mixture.

Optimize the newly developed method for speed.

Participants will end up with a method that runs in less than 2 minutes…for a baseline separation of 11 compounds!

 

Course Locations

Date

November 18 - 21, 2014

Check-in opens at 8:00 a.m. on the first day of the course.

Course runs from 8:30 a.m. to 4:30 p.m. each day

Register Online Register Via Mail

Venue

Axion Analytical Laboratories
14 N Peoria Street, Suite 100
Chicago, IL 60607

The course fee includes tuition, lecture and laboratory materials, morning and afternoon snacks, and all lunches.


Pricing
  Member Non-Member
Standard $3,595 $3,795

View complete details about lodging, local dining options, and available transportation.

About the Instructors

  • Lee N. Polite

    is the President and Laboratory Director, Axion Analytical Laboratories.

  • Harold McNair

    is Professor Emeritus of Chemistry, Virginia Tech, Blacksburg, Virginia.