Course Catalog

High Performance Liquid Chromatography Basics OnDemand

This course will help you gain a sound working knowledge of basic, high-performance liquid chromatographic principles and develop a greater understanding of theoretical and practical relationships. Build the expertise you need to choose optimized column parameters, detector selection, and separation mechanism for the examination of complex mixtures, and improve your on-the-job performance.

Course Details

Key Topics

  • HPLC theory and best practices
  • Column optimization
  • Detector selection
  • Improve separation of complex mixtures

Information

This course will help you gain a sound working knowledge of basic, high-performance liquid chromatographic principles and develop a greater understanding of theoretical and practical relationships. Build the expertise you need to choose optimized column parameters, detector selection, and separation mechanism for the examination of complex mixtures, and improve your on-the-job performance.

Who Should Attend

Ideal for R&D scientists and technicians or anyone who uses HPLC and wishes to have a solid theoretical and practical background in this technique.

Benefits

  • Move beyond standard operating procedures, and gain a solid working knowledge of HPLC principles and methods.
  • Build expertise that will help you choose the right column, conditions, detector, and separation mechanism to separate difficult mixtures.
  • Improve your on-the-job performance and skill set.

Agenda

Please note: Module #1 and Module #2 are sold together for a single fee of $199.

Module #1 – Introduction

  • Introduction to HPLC
  • Separation Mechanisms
  • Components of HPLC Instrumentation
  • Commercial HPLC Instruments

Module #2 – Theory of HPLC

  • Selection of Separation Mechanism
  • Features of HPLC Chromatograms
  • Resolution of HPLC Columns
  • Resolution vs. Selectivity

Module #3 – Instrumentation

  • Pumps
  • Columns
  • Detectors
  • Ultraviolet
  • Fluorescence
  • Mass Spectrometry
  • Refractive Index
  • Evaporative Light Scattering

Module #4 – Separation Mechanisms

  • Adsorption
  • Partition
  • Normal Phase
  • Reverse Phase
  • Anionic and Cationic Ion Exchange
  • Steric Exclusion
  • Affinity

Module #5 – Qualitative Analysis

  • Retention Time
  • Retention Indices
  • Ultraviolet Spectroscopy
  • Infrared Spectroscopy
  • Mass Spectroscopy

Module #6 – Quantitative Analysis

  • Integration Methods
  • Area Percent
  • Use of Response Factors
  • Internal Standard
  • External Calibration
  • Method of Additions

Module #7 – Alternate and Optimization Techniques

  • Enhanced UV Detection
  • Capillary Zone Electrophoresis
  • Octanol / Water Partition Coefficients
  • Gradient Optimization
  • Methods Development Criteria
  • Computer Based Optimization

Course Locations

Date

TBA

This course is available on demand

This course consists of six modules which can be purchased separately. Each module is approximately 60 minutes long. Modules #1 and #2 are sold together for a single fee.

Register Online Register Via Mail

Venue

Online

The registration fee includes seven days of access to the content, lecture slides (provided in PDF format), and email access to the instructor.

Each person attending must register individually for this course.


Pricing
Standard $199 per module

About the Instructor

  • Dennis Anderson

    retired from Akzo Nobel Surfactants America following a 40+-year career as an industrial analytical chemist and taught HPLC Basics OnDemand.