Course Catalog

High Performance Liquid Chromatography: Fundamentals, Troubleshooting, and Method Development

This course is designed for beginning- and intermediate-level users in HPLC who want practical laboratory experience. The lectures, supplemented by problems sets, slides, and video presentations, provide the fundamentals needed to understand the techniques and instrumentation involved in this powerful analytical tool. Key topics include: basic instrumentation for HPLC; detectors, including UV/VIS, photo diode array, mass spectrometer, and fluorescence; column selection and optimization, qualitative and quantitative analysis; and troubleshooting HPLC systems.

Course Details

Key Topics

  • Basic instrumentation for HPLC
  • Column selection and optimization
  • Detectors: UV/VIS, photo diode array, mass spectrometer, fluorescence
  • Qualitative and quantitative analysis
  • Troubleshooting HPLC systems

Information

This course is designed for beginning- and intermediate-level users in HPLC who want practical laboratory experience. The lectures, supplemented by problems sets, slides, and video presentations, provide the fundamentals needed to understand the techniques and instrumentation involved in this powerful analytical tool. Key topics include: basic instrumentation for HPLC; detectors, including UV/VIS, photo diode array, mass spectrometer, and fluorescence; column selection and optimization, qualitative and quantitative analysis; and troubleshooting HPLC systems.

Who Should Attend

This course is designed for beginners and intermediate-level users in HPLC who want practical laboratory experience.

The lectures, supplemented by problems sets, slides, and video tapes, provide the fundamentals needed to understand the techniques and instrumentation involved in this powerful analytical tool. No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course.

Some experience in HPLC or introductory course work in organic chemistry is desirable but not necessary.

Benefits

  • Learn about basic instrumentation for HPLC.
  • Learn about detectors: UV/VIS, PDA, RI, and Fluorescence.
  • Become familiar with column selection and optimization.
  • Perform qualitative and quantitative analysis.
  • Understand how to troubleshoot HPLC systems.

Agenda

  • HPLC Overview–introduction to HPLC; instrumentation overview; detector details; Laboratory: basic HPLC hardware.
  • Separation Fundamentals and Mobile Phase–practical HPLC theory; important HPLC parameters; the mobile phase; Laboratory: mobile phase strength flow rate and temperature.
  • Separation Modes and Columns–reversed phase; normal phase; ion exchange; size exchange; Laboratories: pH effects in reversed phase and column dimensions.
  • Quantitative Analysis and Troubleshooting–qualitative and quantitative analysis; LC/MS; hardware troubleshooting; separation troubleshooting; Laboratory: internal vs. external standard; hardware troubleshooting.
  • Gradients and Method Development–gradients; fundamentals of method development; Laboratory: develop a method from a completely unknown mixture.

Course Locations

Date

TBA

Check-in opens at 8:00 a.m. on the first day of the course.

Course runs from 8:30 a.m. to 4:30 p.m. each day

Register Online Register Via Mail

Venue

The course fee includes tuition, lecture and laboratory materials, morning and afternoon snacks, and all lunches.


Pricing
  Member Non-Member
Standard $3,595 $3,795

View complete details about lodging, local dining options, and available transportation.

About the Instructors

  • Lee N. Polite

    is the President and Laboratory Director, Axion Analytical Laboratories.

  • Harold McNair

    is Professor Emeritus of Chemistry, Virginia Tech, Blacksburg, Virginia.