Course Catalog

Saving Time and Money with 2D Liquid Chromatography

One-dimensional liquid chromatography remains useful, however functional limitations can result in costly and timely methods. 2D liquid chromatography offers greater flexibility and substantial workflow efficiencies. This new course offering for practicing analytical chemists and separation scientists demonstrates the vast differences between 1D and 2D HPLC, both in terms of hardware and protocols. Attendees will leave this course with documented examples of how 2D liquid chromatography can improve methods and workflow.

Course Details

Key Topics

  • Limitations of 1D workflow protocols
  • Advantages of 2D liquid chromatography
  • 2D liquid chromatography configuration
  • Advanced 2D configuration and parameters
  • Method optimization and automation
  • Troubleshooting 2D liquid chromatography


Multi-dimension chromatography is a separation technique with a long history. Originally developed to increase resolution power for complex extracts, the first versions were mostly operated with manual controls and where restricted to academic labs. The main problem in those early days was triggering flow streams (pumps) and flow direction (valves) with precise timing during the chromatographic separation. From those experiments, multi-dimension chromatography was perceived as a difficult technique to utilize and a complex one to understand. With full software control, multi-dimension chromatography is rapidly gaining interest and can offer a plethora of new options not available with current single dimension chromatographic solutions. With novel injection capabilities and fluidic circuits, multi-dimension chromatography brings cost effective workflow protocols to both industry and academic applications.

This 4 hour course is stand alone, but also represents the first ½ of “Workflow Efficiencies: 2D Liquid Chromatography and Novel Sample Preparation Techniques”, which additionally presents how sample preparation techniques adjusted for 2D HPLC can add efficiency to your overall workflow protocol. This full day course is also being offered in Boston.

Who Should Attend

This course is designed for practicing separation scientists and chromatographers from all sectors, including but not limited to Clinical, Forensic, Pharmaceutical, Environmental, Food Safety, Energy, and Natural Products. Participants are expected to be knowledgeable in the theory and practice of liquid chromatography.


Learning objectives:

  • Identify the limitations of 1D chromatography workflow processes
  • Outline the fundamental differences between 1D and 2D liquid chromatography
  • Recognize current method inefficiencies
  • Document ways to increase workflow efficiency via 2D liquid chromatography.
  • Recognize issues that may arise in 2D LC configurations



  • Observations of current 1D workflow protocols
  • Is multi-dimensional chromatography complex?

Current HPLC workflow protocols

  • Sector independent examples (1D)
  • Are they optimized and efficient?
  • What limitations exist in 1D liquid chromatography

Basic 2D Configuration

  • Analytical Columns vs. Trap Columns
  • Key Elution Parameters
  • Transitioning from 1D to 2D – hardware
  • 1D configuration, but still limitations

Advanced 2D Configuration

  • AT-Column Dilution
  • Aqueous Extracts vs Organic Extracts

Automated Method Optimization

  • Moving from theory to implementation
    • 3×3 – 4.5 hrs
    • 3×6 – 9 hrs
    • 6×6 – 18 hrs


  • Leading & Tailing Peaks
  • Shoulder Peaks (Left & Right)
  • Adsorption Effects
  • Dilution rate

Course Locations



  Member Non-Member
Standard 550.00 650.00

About the Instructor

  • Dr. Claude Mallet

    Dr. Mallet received his Ph.D. in analytical chemistry from the University of Montreal, Montreal, Canada (1997). His thesis was entitled “Time-Coupled-timed-Resolved Chromatography or TCRC”.