Course Catalog

Workflow Efficiencies: 2D Liquid Chromatography and Novel Sample Preparation Techniques

Workflow efficiency can be realized through 2D liquid chromatography techniques and associated novel sample preparation approaches. Learners will receive guidance on 2D hardware parameters and gain insight and implementable tools covering novel micro extraction process and disruptive concepts such as: elimination of evaporation & reconstitution steps, high volume injection, and analysis of clean organic extracts. Attendees will leave this day-long course with cost and time saving measures that can be immediately applied to their own lab protocols.

Course Details

Key Topics

2D liquid chromatography

  • Limitations of 1D workflow protocols
  • Advantages of 2D liquid chromatography
  • 2D liquid chromatography configuration
  • Advanced 2D configuration and parameters
  • Method optimization and automation
  • Troubleshooting 2D liquid chromatography

Sample preparation

  • Sample preparation for hplc
  • 1D Extraction Protocols
  • 2D Extraction Protocols
  • Realizing workflow efficiencies – case study
  • Implementing protocols


The concept of multi-dimension chromatography with AT-column dilution offers the option of large volume injection and clean organic extracts. These new hardware capabilities bring options in developing cost effective sample preparation protocols. The enrichment requirement for trace analysis is no longer a requirement within a sample preparation protocol, and can now be shared with the 2D LC solution by using large volume injection (up to 1000 uL). This then allows exploration of micro extraction protocols that convert day-long extraction process to less than one hour. As an added bonus, the 2D LC with AT-column dilution also brings a disruptive contribution that bolsters efficiency. With the added dilution, clean organic extracts can also be considered for analysis. This eliminates the time consuming evaporation and reconstitution steps within the extraction protocols created for 1D chromatography.

Who Should Attend

This course is designed for practicing separation scientists and chromatographers from all sectors, including but not limited to Clinical, Forensic, Pharmaceutical, Environmental, Food Safety, Energy, and Natural Products. Participants are expected to be knowledgeable in the theory and practice of liquid chromatography and familiar with standard sample preparation techniques.


Learning objectives:

  • Identify the limitations of 1D chromatography workflow processes
  • Outline the fundamental differences between 1D and 2D liquid chromatography
  • Recognize current method inefficiencies
  • Document ways to increase workflow efficiency via 2D liquid chromatography
  • Recognize how 1D vs. 2D sample preparation techniques differ
  • Explore novel sample preparation techniques that enhance workflow efficiencies
  • Investigate disruptive sample preparation techniques aligned with 2D liquid chromatography


Morning Session

  • Observations of current 1D workflow protocols
  • Is multi-dimensional chromatography complex?

Current HPLC workflow protocols

  • Sector independent examples (1D)
  • Are they optimized and efficient?
  • What limitations exist in 1D liquid chromatography

Basic 2D Configuration

  • Analytical Columns vs. Trap Columns
  • Key Elution Parameters
  • Transitioning from 1D to 2D – hardware
  • 1D configuration, but still limitations

Advanced 2D Configuration

  • AT-Column Dilution
  • Aqueous Extracts vs Organic Extracts

Automated Method Optimization

  • Moving from theory to implementation
    • 3×3 – 4.5 hrs
    • 3×6 – 9 hrs
    • 6×6 – 18 hrs


  • Leading & Tailing Peaks
  • Shoulder Peaks (Left & Right)
  • Adsorption Effects
  • Dilution rate

Afternoon Session

  • Workflow Associated with Manual Extraction Protocols
  • Cost & Time associated with techniques

1D Extraction Protocols

  • SPE – RP vs IE
  • Class A, B & C matrix
  • Identifying sample preparation techniques – learner examples
  • Parallel column regeneration
  • Limitations of 1D sample preparation techniques

2D Extraction Protocols

  • Micro Sample Size (Volume & Mass)
  • Elimination of the Evaporation/Reconstitution Steps
  • Cumulative vs Sequential Elution

Realizing workflow efficiencies

  • Case study
  • Implementing protocols

Course Locations



8 hour short course

Register Online Register Via Mail



8:30 am – 5:00 pm

  Member Non-Member
Standard 995.00 1195

About the Instructor

  • Dr. Claude Mallet

    Dr. Mallet received his Ph.D. in analytical chemistry from the University of Montreal, Montreal, Canada (1997). His thesis was entitled “Time-Coupled-timed-Resolved Chromatography or TCRC”.