In Person

Fundamentals of High Performance Liquid Chromatography

Gain a sound working knowledge of high performance liquid chromatography methods, techniques, and equipment.

About the Course

Gain a sound working knowledge of high performance liquid chromatography methods, techniques, and equipment. You’ll learn how to use and optimize HPLC columns, and how to interpret and troubleshoot from chromatograms. You’ll also have an opportunity to discuss analytical laboratory problems with recognized experts. Key topics covered include:  

  • Basic instrumentation for HPLC.
  • Detectors, including UV/VIS, photo diode array (PDA), refractive index (RI), fluorescence, and conductivity.
  • Column selection and optimization.
  • Troubleshooting HPLC systems.
  • Qualitative and quantitative analysis.
  • Reversed phase, GPC, ion exchange, and ion chromatography.


What You Will Learn

  • Solutions to your analytical laboratory problems from a recognized expert
  • Modern HPLC methods, techniques, and equipment
  • How to use and optimize HPLC columns
  • How to interpret and troubleshoot from chromatograms

Who Should Attend

Research chemists, biochemists, biologists, technicians, and others with limited knowledge of this important analytical technique.

The course does not require expertise in advanced mathematics, theoretical chemistry, or physics.

Course Outline

  • Topics

    • Introduction to HPLC
    • Instrumentation
    • Detectors
    • Column Selection-adsorption; bonded phases; ion exchange; gel permeation
    • HPLC Troubleshooting
    • HPLC Theory
    • Quantitative Analysis
    • Troubleshooting HPLC Columns
    • Troubleshooting by Diagnosis of Chromatograms

Dates, Locations, and Prices

Five for four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. The free registration will be the course of the lowest price. Please note: This discount is only available if you register by fax, phone, or mail and mention this discount and it cannot be combined with any other offer.

About the Instructor(s)

Lee N. Polite

President , Axion Analytical Labs, Inc

Dr. Lee N. Polite is the president and founder of Axion Analytical Labs, Inc. He has taught chromatography to customers from every major pharmaceutical, chemical, and petroleum company in the U.S.