In Person

HPLC and UHPLC for Practicing Scientists 1: Fundamentals

An all-inclusive one-day course on HPLC and UHPLC fundamentals, including theory, column, instrument, and pharmaceutical applications.

About the Course

This intermediate one-day workshop will provide the analytical scientist with a clearer understanding and solid working knowledge of the concepts, instrumentation, and columns of HPLC and UHPLC (ultra-high-pressure liquid chromatograph). The focus is on pharmaceutical analysis of small molecule drugs.

Key topics include:

  • Modern Concepts
  •  HPLC Columns, Trends and Selection Guides
  •  HPLC instrumentation and Operating Principles

Recommended textbook: M. W. Dong, HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, Hoboken, NJ, 2019

What You Will Learn

  •  Modern theory in HPLC and UHPLC
  •  Fundamentals of HPLC columns, modern trends, and how to select columns for different analysis
  •  An overview of HPLC instrumentation (system, pump, injector, detector, data system) and their operating principles
  • A brief review of the drug development process, regulations, and pharmaceutical analysis of small molecule drugs

Who Should Attend

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of the fundamentals of HPLC and UHPLC in pharmaceutical analysis and other applications.

A basic understanding of chemistry and HPLC with some hands-on experience is assumed. 

Course Outline

  • 1. Modern Concepts

    A. Introduction:  The chromatographic process, advantages and limitations, and chromatographic modes (NPC, RPC, IEC, SEC)

    B.  Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs), tailing factor (Tf), the resolution equation, the linear solvent strength theory, and column void volume (VM

    Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG), column temperature (T)), peak capacity (Pc), references

     

  • 2. HPLC Columns, Trends and Selection Guides 

    A.      Column fundamentals: Glossary and abbreviations, Characteristics and types, packing characteristics (support type, particle size, pore size), bonded phases, end-fittings, plate height (H), the van Deemter equation, and estimation of column efficiency

    B.      Trends: Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, the concept of “orthogonality,” a listing of common RPC columns, column selection guide 

    C.      Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2mm, and superficially porous particles (SPP)

  • 3. HPLC instrumentation and Operating Principles 

    A.      System, Pump, and Autosampler. Integrated vs. modular system, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume and ramification, pump trends, manual injection valve, autosampler types and trends 

    B.      Detectors: Operating principles of UV/Vis detection and characteristics, monitoring wavelength selection,  photodiode array detector (DAD), newer detectors for non-chromophoric analytes (ELSD, CAD, and CLND)

    C.      MS, CDS, peak integration strategy, and Instrument Bandwidth. The mass spectrometer (MS), types and ESI interface, Data Chromatography System (CDS), the concept of instrumental bandwidth (extra-column band broadening), loss of column efficiency vs. column size   

  • 4. Drug Development Process, Regulations, and Pharmaceutical Analysis of Small Molecule Drugs 

    A brief overview of pharmaceutical discovery/development, Chemistry, Manufacturing and Control (CMC), analytical development and quality control in small molecule drugs development, Regulations, potency assay, stability-indicating method, stability study, dissolution testing, chiral separations, and certificates of analysis of drug substance and reference materials, OTC products with multiple actives, herbal products, pharmaceutical counterions and excipients

Dates, Locations, and Prices

Five for four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. The free registration will be the course of the lowest price. Please note: This discount is only available if you register by fax, phone, or mail and mention this discount and it cannot be combined with any other offer.

About the Instructor(s)

Michael W. Dong

Principal Consultant , MWD Consulting

Dr. Michael W. Dong is a principal consultant at MWD Consulting, focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis, and drug quality.