In Person

HPLC and UHPLC for Practicing Scientists 2: Best Practices in Method Development and Operation/Troubleshooting

An all-inclusive, 1-day course on HPLC and UHPLC Method Development, Operation, Maintenance, Troubleshooting, UHPLC Best Practices, Biopharmaceutical and Other Applications

About the Course

This intermediate one-day course will provide the analytical scientist with a clearer understanding and solid working knowledge of best practices (method development, operation, and troubleshooting) of HPLC and UHPLC (ultra-high-pressure liquid chromatograph). The focus is on pharmaceutical analysis of small molecule drugs. A brief survey of biopharmaceutical, food, environmental, chemical, and bioscience applications is included. 

  • HPLC Applications and an Overview of UHPLC
  • HPLC Method Development and Validation
  • HPLC Operation and Troubleshooting

Recommended textbook: M. W. Dong, HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, Hoboken, NJ, 2019

What You Will Learn

  •  An overview of HPLC applications, including food, environmental, industrial, GPC, and biopharmaceutical analysis
  • Concepts, perspectives, best practices, and potential issues surrounding UHPLC
  •  Fundamentals of HPLC method development and easier approaches
  •  Overview of method validation and transfer
  •  Standard operating procedures for HPLC modules, and troubleshooting strategies

Who Should Attend

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of fundamentals and best practices of method development, troubleshooting, UHPLC, and diversified HPLC applications. 

A basic understanding of chemistry and HPLC with some hands-on experience is assumed.

Course Outline

  • HPLC Applications and an Overview of UHPLC

    A.    HPLC applications including pharmaceutical, biopharmaceuticals, food, environmental, chemical, and bioseparations

    B.    UHPLC overview, perspectives, and benefits

    C.     Potential issues and how to mitigate

    D.     Chemical: GPC, plastics, and ion chromatography.

    E.     Bioseparations and life sciences: proteins, peptides, peptide mapping, amino acids, oligonucleotides, nucleic acids, and PCR products.

  • UHPLC: Concept, Perspectives, Practice and Potential Issue

    A.      Overview and perspectives, UHPLC concepts (the need for high pressure, void volume, peak volume, instrumental bandwidth), UV flow cell design, dwell volume

    B.      Benefits and case studies: very fast separations with good resolution, very high-resolution analysis of complex samples, facilitating rapid method development, flexibility to customize resolution

    C.      Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC and vice versa using geometric scaling, how to transition from HPLC to UHPLC, end-fittings

  • HPLC Method Development and Validation

    A.      Tradition strategy and a survey of automated tools 

     

    B.      3-pronged template approach 

    C.      Use of a universal generic gradient method 

    D.      Method validation and method transfer

  • HPLC Operation and Troubleshooting

    A.      Mobile phase and sample preparation and  HPLC operation

    B.      Maintenance and troubleshooting guide

    C.   Diagnosing and solving problems

Dates, Locations, and Prices

*Early bird pricing ends four (4) weeks before the start date of the course.

Five for four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. The free registration will be the course of the lowest price. Please note: This discount is only available if you register by fax, phone, or mail and mention this discount and it cannot be combined with any other offer

About the Instructor(s)

Michael W. Dong

Principal Consultant , MWD Consulting

Dr. Michael W. Dong is a principal consultant at MWD Consulting, focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis, and drug quality.