Gain practical laboratory experience in gas chromatography. The course provides the fundamentals to understand the techniques and instrumentation involved in this powerful analytical tool.
This course is designed for beginners and intermediate-level practitioners who want practical laboratory experience in gas chromatography (GC).
The lectures—supplemented by problem sets, slides, and video presentations—provide the fundamentals needed to understand the technique and instrumentation involved in this powerful analytical tool. In this class, you will master the fundamentals of GC, participate in five hands-on laboratory sessions, perform reference and literature searches, and learn specialized techniques based on your specific interests.
Beginners and intermediate-level practitioners in gas chromatography who want practical laboratory experience.
No previous experience in gas chromatography is required for the course.
Day 1: Gas Chromatography (GC) Overview & Injection Techniques
Introduction to GC and instrumentation
The GC Separation: How it occurs
Packed & Capillary columns
Capillary inlet systems: split, splitless, on-column
Laboratory Experiments:
GC Familiarization / Temperature effects
Split ratio
Comparing split and splitless injection techniques
Laboratory Experiments:
GC Familiarization / Temperature effects
Split ratio
Comparing split and splitless injection techniques
Day 2: Resolution, Columns and Quantitative Analysis
Resolution Equation (how to choose the correct chromatographic conditions)
GC Theory Overview – Explains why we like small diameters and thin films
Capillary column technology; column dimension and stationary phase selection
Calibration methods: Area % – internal and external standard
Laboratory Experiments:
GC Parameters
Detailed investigation of temperature and flow effects on a separation
Day 3: Detectors, GC/MS, Headspace
Overview of available detectors; details of FID & TCD
GC/MS – How it works and why it is important
Headspace GC – Theory, practice and instrumentation
Laboratory Experiments:
Column installation
Injector disassembly and maintenance
Quantitation of an unknown employing internal vs. external standard techniques
Day 4: Method Development & Troubleshooting/Maintenance
Temperature programming
Fundamentals of method development
Troubleshooting Fundamentals
Troubleshooting from actual chromatograms
Laboratory Experiments:
Develop a method for a completely unknown mixture.
Optimize the newly developed method for speed.
Participants will end up with a method that runs in less than 2 minutes…for a baseline separation of 11 compounds!
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Dr. Lee N. Polite is the president and founder of Axion Analytical Labs, Inc. He has taught chromatography to customers from every major pharmaceutical, chemical, and petroleum company in the U.S.
