Lab Component

High Performance Liquid Chromatography: Fundamentals, Troubleshooting, and Method Development

Gain practical laboratory experience in HPLC and learn the fundamentals needed to understand the techniques and instrumentation involved in this powerful analytical tool.

Chicago, IL
Apr 23 - Apr 26, 2019
Chicago, IL
Oct 01 - Oct 04, 2019
Prices start at
$2995
Registration Info

About the Course

Youtube ID: TQbWtrcim3A


This course is designed for beginning- and intermediate-level users in HPLC who want practical laboratory experience. Lectures are supplemented by problem sets, slides, and video presentations.

Key topics include: basic instrumentation for HPLC; detectors, including UV/VIS, photo diode array, mass spectrometer, and fluorescence; column selection and optimization; qualitative and quantitative analysis; and troubleshooting HPLC systems

  •  HPLC Overview–introduction to HPLC; instrumentation overview; detector details
    Laboratory: basic HPLC hardware familiarization
  • Separation Fundamentals and Mobile Phase–practical HPLC theory; important HPLC parameters; the mobile phase
    Laboratory: mobile phase strength, flow rate, and temperature
  • Separation Modes and Columns–reversed phase; normal phase; ion exchange; size exchange
    Laboratories: pH effects in reversed phase and column dimensions
  • Quantitative Analysis and Troubleshooting–qualitative and quantitative analysis; LC/MS; hardware troubleshooting; separation troubleshooting 
    Laboratory: internal vs. external standard; hardware troubleshooting.
  • Gradients and Method Development–gradients; fundamentals of method development
    Laboratory: develop a method to separate a completely unknown mixture.

 

What You Will Learn

Each attendee will leave the course with the ability to:

  • Understand how each HPLC parameter affects separation
  • Build a calibration table to quantify samples
  • Develop and optimize HPLC methods from scratch
  • Cut analysis times in half
  • Speak as an authority in chromatography
  • Troubleshoot and fix HPLC issues

Who Should Attend

Beginners and intermediate-level HPLC users who want practical laboratory experience.

No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course.

Some experience in HPLC or introductory course work in organic chemistry is desirable but not necessary.

Course Outline

  • Topics Covered

    Introduction and Definitions

    Instrumentation

    Resolution

    HPLC Theory

    Detectors

    HPLC Columns

    Reversed Phase of Ionic Samples

    Quantitative Analysis

    Normal Phase

    Hydrophilic Interaction (HILIC)

    Ion Exchange Chromatography

    Size Exclusion Chromatography

    Method Development

    Preventative Maintenance

    Troubleshooting Exercises

    Read complete details about lodging, local dining options, and available transportation.

Dates, Locations, and Prices

Lab Component

Apr 23 - Apr 26, 2019

Axion Analytical Labs
14 N Peoria Street, Suite 100
Chicago, IL 60607

Standard Member $2995 Non-Member $3195
Become a member during registration
- Less Details+ More Details

Course fee includes tuition, lecture and laboratory materials, morning and afternoon snacks, and all lunches.

Lab Component

Oct 01 - Oct 04, 2019

Axion Analytical Labs
14 N Peoria Street, Suite 100
Chicago, IL 60607

Standard Member $2995 Non-Member $3195
Become a member during registration
- Less Details+ More Details

Course fee includes tuition, lecture and laboratory materials, morning and afternoon snacks, and all lunches. View complete details about lodging, local dining options, and available transportation here.

About the Instructor(s)

Lee N. Polite

President , Axion Analytical Labs, Inc

Dr. Lee N. Polite is the president and founder of Axion Analytical Labs, Inc. He has taught chromatography to customers from every major pharmaceutical, chemical, and petroleum company in the U.S.

Chicago, IL
Apr 23 - Apr 26, 2019
Chicago, IL
Oct 01 - Oct 04, 2019
Prices start at
$2995
Registration Info