Gain practical laboratory experience in HPLC and learn the fundamentals needed to understand the techniques and instrumentation involved in this powerful analytical tool.
This course is designed for beginning- and intermediate-level users in HPLC who want practical laboratory experience. Lectures are supplemented by problem sets, slides, and video presentations.
Key topics include: basic instrumentation for HPLC; detectors, including UV/VIS, photo diode array, mass spectrometer, and fluorescence; column selection and optimization; qualitative and quantitative analysis; and troubleshooting HPLC systems
Each attendee will leave the course with the ability to:
Beginners and intermediate-level HPLC users who want practical laboratory experience.
No prior experience in advanced mathematics, theoretical chemistry, or physics is required for this course.
Some experience in HPLC or introductory course work in organic chemistry is desirable but not necessary.
Topics Covered
Introduction and Definitions
Instrumentation
Resolution
HPLC Theory
Detectors
HPLC Columns
Reversed Phase of Ionic Samples
Quantitative Analysis
Normal Phase
Hydrophilic Interaction (HILIC)
Ion Exchange Chromatography
Size Exclusion Chromatography
Method Development
Preventative Maintenance
Troubleshooting Exercises
View complete details about lodging, local dining options, and available transportation.
Dr. Lee N. Polite is the president and founder of Axion Analytical Labs, Inc. He has taught chromatography to customers from every major pharmaceutical, chemical, and petroleum company in the U.S.
