An all-inclusive 6-hour online course on HPLC and UHPLC fundamentals including theory, column, and instrument.
This intermediate course will provide the analytical scientist with a clearer understanding and solid working knowledge of the concepts, instrumentation, and columns of HPLC and UHPLC (ultra-high-pressure liquid chromatograph). The focus is on pharmaceutical analysis of small molecule drugs.
Recommended textbook: M. W. Dong, HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, Hoboken, NJ, 2019
An overview of HPLC instrumentation (system, pump, injector, detector, data system) and their operating principles
Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of the fundamentals of HPLC and UHPLC in pharmaceutical analysis and other applications. A basic understanding of chemistry and HPLC with some hands-on experience is assumed.
Introduction: The chromatographic process, advantages and limitations, and chromatographic modes (NPC, RPC, IEC, SEC)
Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs), tailing factor (Tf), the resolution equation, the linear solvent strength theory, and column void volume (VM)
Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG), column temperature (T)), peak capacity (Pc), references
HPLC Columns, Trends and Selection Guides
Column fundamentals: Glossary and abbreviations, Characteristics and types, packing characteristics (support type, particle size, pore size), bonded phases, end-fittings, plate height (H), the van Deemter equation, and estimation of column efficiency
Trends: Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, the concept of “orthogonality,” a listing of common RPC columns, column selection guide
Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2mm, and superficially porous particles (SPP)
HPLC instrumentation and Operating Principles
System, Pump, and Autosampler. Integrated vs. modular system, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume and ramification, pump trends, manual injection valve, autosampler types and trends
Detectors: Operating principles of UV/Vis detection and characteristics, monitoring wavelength selection, photodiode array detector (DAD), newer detectors for non-chromophoric analytes (ELSD, CAD, and CLND)
MS, CDS, peak integration strategy, and Instrument Bandwidth. The mass spectrometer (MS), types and ESI interface, Chromatography Data System (CDS), the concept of instrumental bandwidth (extra-column band broadening), loss of column efficiency vs. column size
Five for four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. The free registration will be the course of the lowest price. Please note: This discount cannot be combined with any other discount offered.
Each person attending must register individually for this course.
Course fee includes electronic access to the course materials and session recordings.
To complete your registration, you will be asked to log in using your ACS ID. If you are not already logged in, you may enter your credentials or create an ID from the next screen.
Dr. Michael W. Dong is a principal consultant at MWD Consulting, focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis, and drug quality.