Intermediate-level course on HPLC and UHPLC Method Development, Operation, Maintenance, Troubleshooting, and UHPLC Best Practices.
(Intermediate Level Workshop) Gain a clearer understanding and a solid working knowledge on method development, Ultra High Performance Liquid Chromatography (UHPLC) best practices, High Performance Liquid Chromatography (HPLC) operations and troubleshooting, and Biopharmaceutical and other applications.
Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of fundamentals and best practice of method development, troubleshooting, and UHPLC.
A basic understanding of chemistry and HPLC with some hands-on experience is assumed.
UHPLC: Concept, Perspectives, Practice and Potential Issues
A. Overview and perspectives, UHPLC concepts (the need for high pressure, void volume, peak volume, instrumental bandwidth), UV flow cell design, dwell volume
Benefits and case studies: very fast separations with good resolution, very high-resolution analysis of complex samples, facilitating rapid method development, flexibility to customize resolution
B. Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC and vice versa using geometric scaling, how to transition from HPLC to UHPLC, end-fittings
HPLC Method Development and Validation
A. Tradition strategy for HPLC method development
B. 3-pronged template approach for rapid method development
C. Use of a universal generic gradient method for the assay of multiple NCEs and stability-indicating assays with simple adjustments
D. A brief overview of method validation and transfer with case studies.
HPLC Operation and Troubleshooting
A. Mobile phase and sample preparation and best practice in HPLC operation
B. Problem diagnosis and troubleshooting guide with case studies
C. Diagnosing and solving problems (pressure, baseline, peak, data performance)
Five for four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. The free registration will be the course of the lowest price. Please note: This discount is only available if you register by fax, phone, or mail and mention this discount and it cannot be combined with any other offer.
Dr. Michael W. Dong is a principal consultant at MWD Consulting, focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis, and drug quality.