Intermediate-level course on HPLC and UHPLC Method Development, Operation, Maintenance, Troubleshooting, and UHPLC Best Practices.
This intermediate one-day workshop will provide the analytical scientist with a clearer understanding and solid working knowledge of best practices (method development, operation, and troubleshooting) of HPLC and UHPLC (ultra-high-pressure liquid chromatograph). The focus is on pharmaceutical analysis of small molecule drugs. A brief survey of biopharmaceutical, food, environmental, chemical, and bioscience applications is included.
Recommended textbook: M. W. Dong, HPLC and UHPLC for Practicing Scientists, 2nd Ed., Wiley, Hoboken, NJ, 2019
Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of the best practices of HPLC and UHPLC in pharmaceutical analysis including HPLC method development, operation, and troubleshooting. A basic understanding of chemistry and HPLC with some hands-on experience is assumed.
HPLC Applications and an Overview of UHPLC
A. Overview and perspectives, UHPLC concepts (the need for high pressure, void volume, peak volume, instrumental bandwidth), UV flow cell design, dwell volume
Benefits and case studies: very fast separations with good resolution, very high-resolution analysis of complex samples, facilitating rapid method development, flexibility to customize resolution
B. Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes), method translation from HPLC to UHPLC and vice versa using geometric scaling, how to transition from HPLC to UHPLC, end-fittings
C. HPLC applications including pharmaceutical, biopharmaceuticals, food, environmental, chemical, and bioseparations
E. UHPLC overview, perspectives, and benefits
F. Potential issues and how to mitigate
HPLC Method Development and Validation
A. Tradition strategy and a survey of automated tools
B. 3-pronged template approach
C. Use of a universal generic gradient method
D. Method validation and method transfer
HPLC Operation and Troubleshooting
A. Mobile phase and sample preparation and best practice in HPLC operation
B. Problem diagnosis and troubleshooting guide with case studies
C. Diagnosing and solving problems (pressure, baseline, peak, data performance)
D. Mobile phase and sample preparation and HPLC operation
E. Maintenance and troubleshooting guide
F. Diagnosing and solving problems
Five for four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. The free registration will be the course of the lowest price. Please note: This discount is only available if you register by fax, phone, or mail and mention this discount and it cannot be combined with any other offer.
Online
This course will meet online for 4 sessions on Wednesday from 10:30 AM - 12: 30 PM ET. Course fee includes electronic access to the course materials and session recordings. Each person attending must register individually for this course.
To complete your registration, you will be asked to log in using your ACS ID. If you are not already logged in, you may enter your credentials or create an ID from the next screen.
Online
This course will meet online for 4 sessions on Wednesday from 10:30 AM - 12: 30 PM ET. Course fee includes electronic access to the course materials and session recordings. Each person attending must register individually for this course.
To complete your registration, you will be asked to log in using your ACS ID. If you are not already logged in, you may enter your credentials or create an ID from the next screen.
Dr. Michael W. Dong is a principal consultant at MWD Consulting, focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis, and drug quality.
