Course Catalog

Modern HPLC/UHPLC for Practicing Scientists 1: Fundamentals Online Short Course

This intermediate 3-session webinar will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, and pharmaceutical applications of modern HPLC and UHPLC (ultra-high-pressure liquid chromatography). The focus is on its practice in pharmaceutical analysis of small molecule drug substances and drug products.

Course Details

Key Topics

Modern (u)HPLC 1: Fundamentals

 

Modern Concepts

 

  1. Introduction: The chromatographic process, advantages and limitations, and chromatographic modes (NPC, RPC, IEC, SEC)
  2. Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs), tailing factor (Tf), the resolution equation, the linear solvent strength theory, and column void volume (VM)
  3. Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG), column temperature (T)), peak capacity (Pc), references

HPLC Columns, Trends and Selection Guides

  1. Column fundamentals: Glossary and abbreviations, Characteristics and types, packing characteristics (support type, particle size, pore size), bonded phases, end-fittings, plate height (H), the van Deemter equation, and estimation of column efficiency
  2. Trends: Trends in shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, the concept of “orthogonality”, a listing of common RPC columns, column selection guide
  3. Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2mm, and superficially porous particles (SPP)

 

HPLC instrumentation and Operating Principles

  1. System, Pump, and Integrated vs. modular system, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume and ramification, pump trends, manual injection valve, autosampler types and trends
  2. Detectors: Operating principles of UV/Vis detection and characteristics, monitoring wavelength selection, photodiode array detector (DAD), newer detectors for non-chromophoric analytes (ELSD, CAD, and CLND)
  3. MS, CDS, peak integration strategy, and Instrument Bandwidth. The mass spectrometer (MS), types and ESI interface, Data Chromatography System (CDS), the concept of instrumental bandwidth (extra-column band broadening), loss of column efficiency vs. column size

Information

This intermediate 3-session webinar will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, and pharmaceutical applications of modern HPLC and UHPLC (ultra-high-pressure liquid chromatography). The focus is on its practice in pharmaceutical analysis of small molecule drug substances and drug products.

M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006 (ISBN-10: 047172789X).

Who Should Attend

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of fundamentals and best practice of modern HPLC/UHPLC in pharmaceutical analysis. A basic understanding of chemistry and HPLC with some hands-on experience is assumed.

Benefits

The attendees will get a critical overview of the fundamentals of modern HPLC/UHPLC relevant in pharmaceutical analysis including modern concepts, advances in instruments, and latest column trends and selection guide.

Agenda

Modern Concepts

  • Introduction: the chromatographic process, advantages and limitations, and chromatographic modes
  • Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs), tailing factor, the resolution equation, linear solvent strength theory, and column void volume (VM)
  • Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG), column temperature (T)), peak capacity (Pc), and references

HPLC Columns, Trends and Selection Guides

  • Column characteristics and types, packing characteristics (support type, particle size, pore size) and bonded phases, end-fittings, van Deemter equation and estimation of column efficiency
  • Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, concept of “orthogonality”, listing of RPC columns, and column selection guide
  • Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2mm, and superficially porous particles (SPP)

HPLC instrumentation and Operating Principles

  • Integrated vs. modular systems, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume and ramification, modern pump trends, manual injection valve, and autosampler types and trends
  • Detectors: Operating principles of UV/Vis detection and characteristics, wavelength selection, photodiode array detector, newer detectors for non-chromophoric analytes (ELSD, CAD and CLND), applications of CAD
  • Mass spectrometer (MS), types of MS and ESI interface, data handling system (CDS). steps in sample analysis, concept of instrumental bandwidth (extra-column bandbroadening), loss of column efficiency vs. column size

Course Locations

Date

TBA

Each session runs 11:30AM-1:30 PM EST

This course will meet online for 3 sessions from 11:30 am to 1:30 pm ET on the following dates:

Wednesdays
October 3
October 10
October 17

Attendance at scheduled class times is NOT required. Registered participants can attend the course by viewing session recordings.

Register Online Register Via Mail

Venue

Online

Each person attending must register individually for this course

The course fee includes a electronic access to the course materials.

Four for three! Register three people for one online course, one person for three online courses, or any combination in between, and the fourth registration is free! This offer can only be used in conjunction with online registrations and cannot be combined with any other offer. All courses must be completed in the same calendar year. All four registrations must be received by our offices on the same day. Note: This discount is only available if you register by fax, mail or phone and mention this discount.


Pricing
  Member Non-Member
Standard $895 per person $1095 per person

System Requirements
You need a high-speed connection to the Internet and an up-to-date version of your web browser. The audio portion of the live session will be provided via VOIP and US toll-free conference call. VOIP is dependent on the quality and speed of your internet connection so a high-speed connection is strongly recommended. Those choosing to use VOIP should also have a headset or microphone in order to interact with the instructor. If not, we recommend you choose the telephone option, or contact us to purchase a headset for $5.

About the Instructor

  • Michael W. Dong

    is a principal consultant in MWD Consulting focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis and drug quality.