Course Catalog

Modern HPLC/UHPLC for Practicing Scientists 1: Fundamentals and Pharmaceutical Applications

This course will provide you with an updated overview and a solid working knowledge of high-performance liquid chromatography (HPLC). The attendees will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guide, and key applications in various industries. This is the first part of a two-course series for Essential of Modern HPLC/UHPLC. The second part, “Essential of Modern HPLC/UHPLC 2”, is a follow-on course that focuses on the practice of HPLC: operation, maintenance, troubleshooting, method development as well as recent developments of ultra-high-pressure LC.

Recommended Textbook: M. W. Dong, Modern HPLC for Practicing Scientists, Wiley-Interscience, New Jersey, 2006. This book is used as a course reference and supplements the course presentation handouts.

Course Details

Key Topics

  • History, advantages, limitations, and modes
  • HPLC columns, trends and selection guides
  • HPLC instrumentation and operating principles
  • Practical applications of HPLC in diversified industries


This intermediate 1-day workshop will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, and pharmaceutical applications of modern HPLC and UHPLC (ultra-high-pressure liquid chromatography). The focus is on its practice in pharmaceutical analysis of small molecule drug substances and drug products.

Who Should Attend

Analysts, scientists, researchers, and managers in pharmaceutical and other industries who want to get an updated overview of fundamentals and best practice of modern HPLC/UHPLC in pharmaceutical analysis. A basic understanding of chemistry and HPLC with some hands-on experience is assumed.


  • Obtain an update and overview of modern HPLC and UHPLC pertinent to new users or practicing scientists
  • Learn essence of HPLC: modes, corollaries, mobile phase and basic concepts
  • Learn fundamentals in column characteristics and selection guide
  • Learn HPLC instrumentation and operating principles
  • Learn practical applications in diversified industries including Fast LC and UHPLC case studies


Day 1: Modern (u)HPLC 1: Fundamentals and Small Molecule Drug Applications
1. Modern Concepts

  • Introduction: The chromatographic process, advantages and limitations, and chromatographic modes (NPC, RPC, IEC, SEC)
  • Concepts: Retention factor (k), separation factor (a), column efficiency (N), resolution (Rs), tailing factor (Tf), the resolution equation, the linear solvent strength theory, and column void volume (VM)
  • Mobile phase factors (organic modifiers, pH, buffers), mass spec compatibility, isocratic and gradient separations, operating parameters (Flow, gradient time (tG), column temperature (T)), peak capacity (Pc), references

2. HPLC Columns, Trends and Selection Guides

  • Column fundamentals: Glossary and abbreviations, Characteristics and types, packing characteristics (support type, particle size, pore size), bonded phases, end-fittings, plate height (H), the van Deemter equation, and an estimation of column efficiency
  • Trends: Trends of shorter and smaller columns and the use of high-purity silica, traditional and novel bonding chemistries, concept of “orthogonality”, listing of common RPC columns, column selection guide
  • Newer column types: Hybrids, charged surface hybrids, HILIC, sub-3 and sub-2mm, and superficially porous particles (SPP)

3. HPLC instrumentation and Operating Principles

  • System, Pump and Autosampler. Integrated vs. modular system, solvent delivery system, low-pressure vs. high-pressure mixing, dwell volume and ramification, pump trends, manual injection valve, autosampler types and trends
  • Detectors: Operating principles of UV/Vis detection and characteristics, monitoring wavelength selection, photodiode array detector (DAD), newer detectors for non-chromophoric analytes (ELSD, CAD and CLND)
  • MS, CDS, peak integration strategy, and Instrument Bandwidth. Mass spectrometer (MS), types and ESI interface, Data Chromatography System (CDS), concept of instrumental bandwidth (extra-column bandbroadening), loss of column efficiency vs. column size

4. Pharmaceutical Analysis of Small Molecule Drugs
A brief overview of pharmaceutical discovery/development, Chemistry, Manufacturing and Control (CMC), analytical development and quality control in small molecule drugs development, potency assay, stability-indicating method, stability study, dissolution testing, chiral separations, and certificates of analysis of drug substance and reference materials, OTC products with multiple actives, herbal products, pharmaceutical counterions and excipients

Course Locations


April 1, 2019

Check-in opens at 7:30 a.m.

Course runs from 8:30 a.m. to 5:00 p.m.

Register Online Register Via Mail


Orlando, FL

The course fee includes a course binder and a continental breakfast.

Five for Four! Register five people for one course, one person for five courses, or any combination in between and your fifth registration is free. Note: This discount is only available if you register by fax or mail and mention this discount. May not be combined with any other offer.

  Member Non-Member
Standard $895 $1095

About the Instructor

  • Michael W. Dong

    is a principal consultant in MWD Consulting focusing on consulting and training services on HPLC/UHPLC, pharmaceutical analysis and drug quality.