Course Catalog

Gas Chromatography: Fundamentals, Troubleshooting, and Method Development

This course is designed for beginners and intermediate-level practitioners who want practical laboratory experience in gas chromatography (GC). The lectures supplemented by problem sets, slides, and video presentations provide the fundamentals needed to understand the technique and instrumentation involved in this powerful analytical tool. At the end of the class, you will have mastered the fundamentals of GC, participated in five hands-on laboratory sessions, performed reference and literature searches, and learned specialized techniques based on your specific interests.

Course Details

Key Topics

  • Overview of Gas Chromatography
  • Injection Techniques
  • Quantitative Analysis
  • Columns and Detectors
  • Temperature Programming
  • Method Development

Information

This course is designed for beginners and intermediate-level practitioners who want practical laboratory experience in gas chromatography (GC). The lectures, supplemented by problem sets, slides, and video presentations, provide the fundamentals needed to understand the technique and instrumentation involved in this powerful analytical tool. At the end of the class, you will have mastered the fundamentals of GC, participated in five hands-on laboratory sessions, performed reference and literature searches, and learned specialized techniques based on your specific interests.

Who Should Attend

This course is designed for beginners and intermediate-level practitioners in gas chromatography who want practical laboratory experience. The lectures, supplemented by problem sets, slides, and video tapes, provide the fundamentals needed to understand the technique and instrumentation involved in this powerful analytical tool.

No previous experience in gas chromatography is required for the course.

Benefits

  • Master the fundamentals of GC
  • Participate in five hands-on laboratory sessions
  • Perform reference and literature searches
  • Learn specialized techniques based on your specific interests

Agenda

Day 1: GC Overview & Injection Techniques
Introduction to GC and instrumentation
The GC Separation:  How it occurs.
Packed & Capillary columns
Capillary inlet systems: split, splitless, on-column

Laboratory Experiments:
GC Familiarization / Temperature effects
Split ratio
Comparing split and splitless injection techniques

Day 2:  Resolution, Columns and Quantitative Analysis
Resolution Equation (how to choose the correct chromatographic conditions).
GC Theory Overview – Explains why we like small diameters and thin films
Capillary column technology; column dimension and stationary phase selection
Calibration methods:  Area % – internal and external standard

Laboratory Experiments:
GC Parameters
Detailed investigation of temperature and flow effects on a separation

Day 3: Detectors, GC/MS, Headspace
Overview of available detectors; details of FID & TCD;
GC/MS – How it works and why it is important
Headspace GC – Theory, practice and instrumentation

Laboratory Experiments:
Column installation;
Injector disassembly and maintenance;
Quantitation of an unknown employing internal vs. external standard techniques.

Day 4:  Method Development & Troubleshooting/Maintenance
Temperature programming;
Fundamentals of method development;
Troubleshooting Fundamentals
Troubleshooting from actual chromatograms

Laboratory Experiments:
Develop a method for a completely unknown mixture.
Optimize the newly developed method for speed.
Participants will end up with a method that runs in less than 2 minutes…for a baseline separation of 11 compounds!

Course Locations

Date

TBA

Check-in opens at 8:00 a.m. on the first day of the course.

Course runs from 8:30 a.m. to 4:30 p.m. each day

Register Online Register Via Mail

Venue

The course fee includes tuition, lecture and laboratory materials, morning and afternoon snacks, and all lunches.

View complete details about lodging, local dining options, and available transportation.


Pricing
  Member Non-Member
Standard $2,995 $3,195

About the Instructor

  • Lee N. Polite

    is the President and Laboratory Director, Axion Analytical Laboratories.