Lab Component

Advanced Topics in HPLC

Chicago , IL
Aug 24 - Aug 27, 2022
Chicago , IL
Nov 11 - Nov 18, 2022
Prices start at
$3795
Registration Info

About the Course

Advanced Topics in HPLC” offers the ultimate breadth and depth of learning…with 10 new opportunities for hands-on experience.

What You Will Learn

You’ll get a chance to:

  • Utilize HILIC to measure sugar content in adulterated fruit juices
  •  Develop a method to simultaneously measure caffeine, artificial sweeteners and preservatives in soft drinks;
  • Convert HPLC methods to UPLC …and cut the analysis time in half!
  • Identify unknowns with the help from the 3-D capabilities of the Diode Array Detector
  • Analyze Hemp samples to determine cannabinoid concentrations
  •  Tear apart and rebuild a modern HPLC Pump
  • And you’ll get to do all of this while having fun…the Axion way!

Who Should Attend

LC GC Bootcamp recommended for beginners and experts alike, as the blueprint essential for instrument optimization.

Course Outline

  • Day 1

    9:00am Review of HPLC Resolution and Theory

    10:45am Lab 1 – Advanced HPLC Parameters

    12:00pm Lunch (provided by Axion)

    1:00pm Lab Review

    1:30pm Column Hardware

    2:30pm Lab 2 – Column Dimensions

    4:00pm Lab Review

    4:15pm Solid Phase Micro Extraction (SPME)

    5:30pm End of Day

  • Day 2

    9:00am Reversed Phase Mechanism and pH Effects (and HILIC?)

    10:15am Lab 3 – pH Effects – Run pH and Caffeine standards at pH of 5.5, 4.7 and 4.0

    11:30am Quantitative Analysis

    12:00pm Lunch (provided by Axion)

    1:00pm Method Development and Gradients

    2:00pm Lab 4:  Qual/Quant of Soft Drinks

    3:30pm Lab Review

    3:45pm Lab 5 – HILIC HPLC of Sugars

    5:30pm End of Day

  • Day 3

    9:00am Ion Chromatography – Dionex Sugars

    9:30am Lab 6 – Dionex Sugars

    10:00am Sample Preparation

    11:00am Lab 7 Amino Acids by Pre-Column Derivatization

    12:00pm Lunch (provided by Axion)

    1:00pm Lab 8 – Method Optimization

    2:30pm Troubleshooting

    5:30pm End of Day

  • Day 4 

    9:00am Troubleshooting HPLC

    10:30am Lab 9 – Pump Maintenance

    11:30am Lab 10 – Detector and Injector Maintenance

    1:00pm End of Course

    *Extra Discussion Topics and Demonstrations:  LC/MS, SPE Separation of Kool Aid, DAD

Dates, Locations, and Prices

Course fee includes tuition, lecture and laboratory materials, morning and afternoon snacks, and all lunches.

View complete details about lodging, local dining options, and available transportation here.

Check in opens at 9:00 AM on the first day of the course.

Lab Component

Aug 24 - Aug 27, 2022

Axion Analytical Labs
14 N Peoria Street, Suite 100
Chicago , IL 60607

Early Bird Full Price
Member(Premium Package) $3795
Member or Associate(Standard or Basic Package) $3995
Non-Member $3995
- Less Details+ More Details

The course runs from 9:00 AM to 5:30 PM each day, class ends at 3:00 on Friday. Course fee includes tuition, lecture and laboratory materials, morning and afternoon snacks, and all lunches.

To complete your registration, you will be asked to log in using your ACS ID. If you are not already logged in, you may enter your credentials or create an ID from the next screen.

Log in or Register Now

Lab Component

Nov 11 - Nov 18, 2022

Axion Analytical Labs
14 N Peoria Street, Suite 100
Chicago , IL 60607

Early Bird Full Price
Member(Premium Package) $3795
Member or Associate(Standard or Basic Package) $3995
Non-Member $3995
- Less Details+ More Details

The course runs from 9:00 AM to 5:30 PM each day, class ends at 3:00 on Friday. Course fee includes tuition, lecture and laboratory materials, morning and afternoon snacks, and all lunches.

To complete your registration, you will be asked to log in using your ACS ID. If you are not already logged in, you may enter your credentials or create an ID from the next screen.

Log in or Register Now

About the Instructor(s)

Lee N. Polite

President , Axion Analytical Labs, Inc

Dr. Lee N. Polite is the president and founder of Axion Analytical Labs, Inc. He has taught chromatography to customers from every major pharmaceutical, chemical, and petroleum company in the U.S.

Chicago , IL
Aug 24 - Aug 27, 2022
Chicago , IL
Nov 11 - Nov 18, 2022
Prices start at
$3795
Registration Info